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KMID : 1137020080190030162
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Journal of Gynecologic Oncology
2008 Volume.19 No. 3 p.162 ~ p.168
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Expression of the p16(INK4a) and Ki-67 in relation to the grade of cervical intraepithelial neoplasia and high-risk human papillomavirus infection
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Nam Eun-Ji
Kim Young-Tae
Kim Jong-Wook
Kim Sung-Hoon
Kim Jae-Hoon
Kim Sang-Wun
Jang Hyoung-Sun
Kim Jae-Wook
Hong Jong-Wook
Lee Dae-Woo
Jang Si-Young
Yi Sang-Yeop
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Abstract
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Objective: The purposes of this study were to evaluate the expression of p16(INK4a) (referred as to p16) and Ki-67 in cervical intraepithelial neoplasia (CIN), and the correlation between high-risk human papillomavirus (HPV) infection and the above biomarkers.
Methods: We analyzed 31 patients who were diagnosed with CIN at Kwandong University Myongji Hospital from October 2006 to September 2007. CIN specimens (CIN1, 12; CIN2, 6; CIN3, 13) were obtained by colposcopy-directed biopsy (CDB) or loop electrical excision procedure (LEEP). The expressions of p16 and Ki-67 were evaluated by immunohistochemical methods with antibodies to p16 and Ki67. The immunohistochemical staining results were classified into four grades: 0, 1, 2 and 3. HPV genotyping or Hybrid Capture-II test was used to detect high-risk HPV.
Results: The expression of p16 (p＜0.001) and Ki-67 (p=0.003) were positively associated with CIN grade. p16 expressions increased significantly with high-risk HPV infection (p=0.014), especially HPV type 16 and 58. Ki-67 expression was not related with high-risk HPV. There was positive correlation between the expression of the p16 and Ki-67 (p=0.007).
Conclusion: CIN grade were positively related to the expression of p16 and Ki-67. p16 expressions of high-risk HPV specimens significantly increased more than Ki-67. Therefore, in the diagnosis of CIN and high-risk HPV infection, p16 can be a useful biomarker.
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KEYWORD
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p16, Ki-67, HPV 16, HPV 58, Cervical intraepithelial neoplasia
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